Water-Based Crystallization
and
Formulation of Stevioside from Stevia rebaudiana
(Bert.) As Natural Sweetener With Antidiabetic Activity
ABSTRACT
Stevioside
is compound that can be extracted from the Stevia rebaudiana (Bert.). The
steviol glycosides of stevoside have potential, functions, and characteristics as natural sweetener. The purposes of this
experiment were to optimize the crystallization method of stevioside, to
indentify stevioside in crystall, to determine the content of stevioside by
using High Performance Liquid Chromatography (HPLC), and to determine
organoleptic assay by hedonic test from crystall formulated. Based on research
obtained the percent yield of each sample was 3,27%, 1,23% (w/w) in stevioside
crystall form, then 3,24%, 4,48%, 6,99% (w/w) in stevioside isolate form. The content of stevioside in
sample 1, 2, 3, 4, 5 were 45,99%, 93,17%, 0,76%, 75,39%, 6,65% (w/w),
respectively. The method developed can be used for stevioside crystallization. Stevioside crystals can be formulated with maltodextrin
as a natural sweetener. Organoleptic
tests showed that the
sweetness of stevioside crystal was more than 100 times sucrose.
In vivo assay using glucose tolerance test showed that lowering blood glucose levels activity of stevioside crystal in low dosage (0.35 mg / kg
bw) was higher
(71.54%) compared with 1.8 mg aspartame / kg bw (36.15% ).
